addgene 535 (Addgene inc)
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addgene 535
Addgene 535, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/addgene+kit/B%2E+subtilis+Strain+Collection+(Kit+%231000000115%2C+1000000116)/pm41298497-300-34-34
Average 93 stars, based on 4 article reviews
Addgene 535, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/addgene+kit/B%2E+subtilis+Strain+Collection+(Kit+%231000000115%2C+1000000116)/pm41298497-300-34-34
Average 93 stars, based on 4 article reviews
addgene 535 - by Bioz Stars,
2026-09
93/100 stars
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other:Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER StemMACSTM Cre Recombinase mRNA Miltenyi Biotec Cat#130-101-113 FIX and PERMTM Cell Permeabilization Kit ThermoFisher Cat#GAS003 E-4031 dihydrochloride Tocris Cat#1808 Verapamil hydrochloride Tocris Cat#0654 Isoprenaline hydrochloride Sigma–Aldrich Cat#I5627 all-trans retinal Sigma–Aldrich Cat#R2500 RNAse A Invitrogen Cat#8003088 Critical commercial assays CloneSmart HCKan Blunt Cloning Kit Sigma–Aldrich Cat#LUC407042 Plasmid-SafeTM ATP-Dependent DNase Biosearch Technologies Cat#E3101K NEBuilder HiFi DNA Assembly Cloning kit New England Biolabs Cat#E5520S NEB PCR Cloning Kit New England Biolabs Cat#E1202S ddPCR Supermix for Probes (no dUTP) Bio-Rad Cat#1863024 QuickExtractTM Biosearch Technologies Cat#QE905T High Pure PCR Template Preparation Kit Roche Cat#11796828001 EnGen sgRNA Synthesis Kit New England Biolabs Cat#E3322V PCR product using theWizard SV Gel and PCR Clean-Up System kit Promega Cat#A9281 NucleoBond Xtra Midi Kit Macherey Nagel Cat#740410.50 NucleoSpin RNA Macherey Nagel Cat#740984.50 DNA-freeTM Kit DNase Treatment and Removal Reagents ThermoFisher Cat#AM1906 iScriptTM cDNA Synthesis Kit Bio-rad Cat#1708891 Deposited data DNA sequencing data This paper https://www.ebi.ac.uk/biostudies/ arrayexpress/studies/E-MTAB-11971 Experimental models: Cell lines LUMC0020iCTRL-06 hiPSC line LUMC hiPSC core facility RRID: CVCL_ZA25 AAVS1_Bxb1 hiPSC line This paper LUMC0020iAAVS-01 AAVS1_4C31 hiPSC line This paper LUMC0020iAAVS-02 AAVS1_Dual hiPSC line This paper LUMC0020iAAVS-03 AAVS1_ASAP2f hiPSC line This paper LUMC0020iAAVS-04 AAVS1_jRCaMP1b hiPSC line This paper LUMC0020iAAVS-05 AAVS1_miRFP703 hiPSC line This paper LUMC0020iAAVS-06 AAVS1_AJMA hiPSC line This paper LUMC0020iAAVS-07 KCNH2+/Acc hiPSC line This paper LUMC0020iHERG-17 KCNH2+/WT hiPSC line clone D1 KCNH2+/WT hiPSC line clone D6 KCNH2+/WT hiPSC line clone G9 This paper LUMC0020iHERG-18 LUMC0020iHERG-19 LUMC0020iHERG-20 KCNH2+/A561T hiPSC line clone D6 KCNH2+/A561T hiPSC line clone E12 KCNH2+/A561T hiPSC line clone F2 This paper LUMC0020iHERG-21 LUMC0020iHERG-22 LUMC0020iHERG-23 Oligonucleotides See Tables S2–S4 Integrated DNA Technologies N/A Recombinant DNA MoClo Toolkit (Weber et al., 2011) Article Title: G z ESTY as an optimized cell-based assay for initial steps in GPCR deorphanization Article Snippet: Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER StemMACSTM Cre Recombinase mRNA Miltenyi Biotec Cat#130-101-113 FIX and PERMTM Cell Permeabilization Kit ThermoFisher Cat#GAS003 E-4031 dihydrochloride Tocris Cat#1808 Verapamil hydrochloride Tocris Cat#0654 Isoprenaline hydrochloride Sigma–Aldrich Cat#I5627 all-trans retinal Sigma–Aldrich Cat#R2500 RNAse A Invitrogen Cat#8003088 Critical commercial assays CloneSmart HCKan Blunt Cloning Kit Sigma–Aldrich Cat#LUC407042 Plasmid-SafeTM ATP-Dependent DNase Biosearch Technologies Cat#E3101K NEBuilder HiFi DNA Assembly Cloning kit New England Biolabs Cat#E5520S NEB PCR Cloning Kit New England Biolabs Cat#E1202S ddPCR Supermix for Probes (no dUTP) Bio-Rad Cat#1863024 QuickExtractTM Biosearch Technologies Cat#QE905T High Pure PCR Template Preparation Kit Roche Cat#11796828001 EnGen sgRNA Synthesis Kit New England Biolabs Cat#E3322V PCR product using theWizard SV Gel and PCR Clean-Up System kit Promega Cat#A9281 NucleoBond Xtra Midi Kit Macherey Nagel Cat#740410.50 NucleoSpin RNA Macherey Nagel Cat#740984.50 DNA-freeTM Kit DNase Treatment and Removal Reagents ThermoFisher Cat#AM1906 iScriptTM cDNA Synthesis Kit Bio-rad Cat#1708891 Deposited data DNA sequencing data This paper https://www.ebi.ac.uk/biostudies/ arrayexpress/studies/E-MTAB-11971 Experimental models: Cell lines LUMC0020iCTRL-06 hiPSC line LUMC hiPSC core facility RRID: CVCL_ZA25 AAVS1_Bxb1 hiPSC line This paper LUMC0020iAAVS-01 AAVS1_4C31 hiPSC line This paper LUMC0020iAAVS-02 AAVS1_Dual hiPSC line This paper LUMC0020iAAVS-03 AAVS1_ASAP2f hiPSC line This paper LUMC0020iAAVS-04 AAVS1_jRCaMP1b hiPSC line This paper LUMC0020iAAVS-05 AAVS1_miRFP703 hiPSC line This paper LUMC0020iAAVS-06 AAVS1_AJMA hiPSC line This paper LUMC0020iAAVS-07 KCNH2+/Acc hiPSC line This paper LUMC0020iHERG-17 KCNH2+/WT hiPSC line clone D1 KCNH2+/WT hiPSC line clone D6 KCNH2+/WT hiPSC line clone G9 This paper LUMC0020iHERG-18 LUMC0020iHERG-19 LUMC0020iHERG-20 KCNH2+/A561T hiPSC line clone D6 KCNH2+/A561T hiPSC line clone E12 KCNH2+/A561T hiPSC line clone F2 This paper LUMC0020iHERG-21 LUMC0020iHERG-22 LUMC0020iHERG-23 Oligonucleotides See Tables S2–S4 Integrated DNA Technologies N/A Recombinant DNA MoClo Toolkit (Weber et al., 2011) Cloning:Article Title: Reproducibly oriented cell divisions pattern the prothallus to set up dorsoventrality and de novo meristem formation in Marchantia polymorpha Article Snippet: .. Escherichia coli : GreenGate Cloning System , Lampropoulos et al. , Plasmid Preparation:Article Title: Inhibitors supercharge kinase turnover through native proteolytic circuits. Article Snippet: The pLEX305-ccdB-Nluc-3×Flag luminescent reporter vector was generated as a destination vector starting from pLEX_305-ccdB-dTAG destination vector (Addgene, 91798) by restriction digest with AgeI and MluI and T4 DNA ligation (NEB) of a synthesized gene block (TWIST) containing the Nluc sequence and a C-terminal 3×Flag tag (5′-CGG GCAAAACCGGTGTCTTCACACTCGAAGATTTCGTTGGGGACTGGCGA CAGACAGCCGGCTACAACCTGGACCAAGTCCTTGAACAGGGAGGTG TGTCCAGTTTGTTTCAGAATCTCGGGGTGTCCGTAACTCCGATCCAA AGGATTGTCCTGAGCGGTGAAAATGGGCTGAAGATCGACATCCATGTC ATCATCCCGTATGAAGGTCTGAGCGGCGACCAAATGGGCCAGATCGA AAAAATTTTTAAGGTGGTGTACCCTGTGGATGATCATCACTTTAAGG TGATCCTGCACTATGGCACACTGGTAATCGACGGGGTTACGCCGAAC ATGATCGACTATTTCGGACGGCCGTATGAAGGCATCGCCGTGTTCGA CGGCAAAAAGATCACTGTAACAGGGACCCTGTGGAACGGCAACAAA ATTATCGACGAGCGCCTGATCAACCCCGACGGCTCCCTGCTGTTCC GAGTAACCATCAACGGAGTGACCGGCTGGCGGCTGTGCGAACGC ATTCTGGCGGACTACAAGGACCACGACGGTGACTACAAGGACCACG ACATCGACTACAAGGACGACGACGACAAGTAGTAAACGCGTTGACGA TGG-3′). .. To generate a destabilized version of the vector, an identical gene block was synthesized with the addition of the PEST sequence (Promega) 5′-AATTCTCACGGCTTTCCGCCTGAGGTTGAAGAGCAAGC CGCCGGTACATTGCCTATGTCCTGCGCACAAGAAAGCGGTATGGACC GGCACCCAGCCGCTTGTGCTTCAGCTCGATCAACGTC-3′ upstream of the stop codon. pENTR223 Gateway entry vectors for the kinases were obtained from Hahn/Root Labs Human Kinases ORF Kit59,60 ( Article Title: The Number of Larval Molts Is Controlled by Hox in Caterpillars. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488-conjugated GFP polyclonal antibody Thermo Fisher Cat#A-21311; RRID: AB_221477 Normal goat serum Sigma-Aldrich Cat#G9023 Biological Samples A genomic DNA panel of B. mori, see Table S1 for details NBRP Silkworm (https://silkworm.nbrp.jp/ index_en.html) N/A Chemicals, Peptides, and Recombinant Proteins TRIzol Reagent Thermo Fisher Cat#15596018 RNeasy Plus Mini Kit QIAGEN Cat#74134 ReverTra Ace qPCR RT Master Mix with gDNA Remover TOYOBO Cat#FSQ-301 Thunderbird SYBR qPCR Mix TOYOBO Cat#QPS-201 mMESSAGE mMACHINE T7 ULTRA Transcription Kit Thermo Fisher Cat#AM1345 TaKaRa Ex Taq TaKaRa Cat#RR001A Methanol Wako Cat#131-01826 alpha-Ecdysone Cayman Chemical Cat#11711 Rhodamine Phalloidin Thermo Fisher Cat#R415 Critical Commercial Assays 20-Hydroxyecdysone EIA Kit Bertin Bioreagent Cat#A05120 Experimental Models: Organisms/Strains B. mori strain p50 (+M) NBRP Silkworm (https://silkworm.nbrp.jp/ index_en.html) p50, a nearly isogenic strain used for whole genome sequencing35 B. mori strain pnd w-1 (+M) Tamura et al.36 N/A, a standard strain for transgenesis B. mori strain Kinshu x Showa (+M) Ueda Sanshu N/A, a commercial hybrid strain used for sericulture B. mori strain Shisen-sanmin (M3) NARO Genebank (https://www.gene.affrc. go.jp/index_en.php) ANJP10282 B. mori strain Kyudai-gomin (M5) NARO Genebank (https://www.gene.affrc. go.jp/index_en.php) ANJP10289 D. melanogaster y w, P[y[+].nos-int.NLS]; P [CaryP]attP2 Gift from Diogo Manoel37 N/A D. melanogaster y w; UAS-mCD8::GFP Bloomington Drosophila Stock Center #5137, RRID:BDSC_5137 Oligonucleotides See Table S2 for primers used in this study N/A Recombinant DNA Golden Gate TALEN and TAL effector kit 2.0 Cermak et al.38 Blocking Assay:Article Title: Inhibitors supercharge kinase turnover through native proteolytic circuits. Article Snippet: The pLEX305-ccdB-Nluc-3×Flag luminescent reporter vector was generated as a destination vector starting from pLEX_305-ccdB-dTAG destination vector (Addgene, 91798) by restriction digest with AgeI and MluI and T4 DNA ligation (NEB) of a synthesized gene block (TWIST) containing the Nluc sequence and a C-terminal 3×Flag tag (5′-CGG GCAAAACCGGTGTCTTCACACTCGAAGATTTCGTTGGGGACTGGCGA CAGACAGCCGGCTACAACCTGGACCAAGTCCTTGAACAGGGAGGTG TGTCCAGTTTGTTTCAGAATCTCGGGGTGTCCGTAACTCCGATCCAA AGGATTGTCCTGAGCGGTGAAAATGGGCTGAAGATCGACATCCATGTC ATCATCCCGTATGAAGGTCTGAGCGGCGACCAAATGGGCCAGATCGA AAAAATTTTTAAGGTGGTGTACCCTGTGGATGATCATCACTTTAAGG TGATCCTGCACTATGGCACACTGGTAATCGACGGGGTTACGCCGAAC ATGATCGACTATTTCGGACGGCCGTATGAAGGCATCGCCGTGTTCGA CGGCAAAAAGATCACTGTAACAGGGACCCTGTGGAACGGCAACAAA ATTATCGACGAGCGCCTGATCAACCCCGACGGCTCCCTGCTGTTCC GAGTAACCATCAACGGAGTGACCGGCTGGCGGCTGTGCGAACGC ATTCTGGCGGACTACAAGGACCACGACGGTGACTACAAGGACCACG ACATCGACTACAAGGACGACGACGACAAGTAGTAAACGCGTTGACGA TGG-3′). .. To generate a destabilized version of the vector, an identical gene block was synthesized with the addition of the PEST sequence (Promega) 5′-AATTCTCACGGCTTTCCGCCTGAGGTTGAAGAGCAAGC CGCCGGTACATTGCCTATGTCCTGCGCACAAGAAAGCGGTATGGACC GGCACCCAGCCGCTTGTGCTTCAGCTCGATCAACGTC-3′ upstream of the stop codon. pENTR223 Gateway entry vectors for the kinases were obtained from Hahn/Root Labs Human Kinases ORF Kit59,60 ( Synthesized:Article Title: Inhibitors supercharge kinase turnover through native proteolytic circuits. Article Snippet: The pLEX305-ccdB-Nluc-3×Flag luminescent reporter vector was generated as a destination vector starting from pLEX_305-ccdB-dTAG destination vector (Addgene, 91798) by restriction digest with AgeI and MluI and T4 DNA ligation (NEB) of a synthesized gene block (TWIST) containing the Nluc sequence and a C-terminal 3×Flag tag (5′-CGG GCAAAACCGGTGTCTTCACACTCGAAGATTTCGTTGGGGACTGGCGA CAGACAGCCGGCTACAACCTGGACCAAGTCCTTGAACAGGGAGGTG TGTCCAGTTTGTTTCAGAATCTCGGGGTGTCCGTAACTCCGATCCAA AGGATTGTCCTGAGCGGTGAAAATGGGCTGAAGATCGACATCCATGTC ATCATCCCGTATGAAGGTCTGAGCGGCGACCAAATGGGCCAGATCGA AAAAATTTTTAAGGTGGTGTACCCTGTGGATGATCATCACTTTAAGG TGATCCTGCACTATGGCACACTGGTAATCGACGGGGTTACGCCGAAC ATGATCGACTATTTCGGACGGCCGTATGAAGGCATCGCCGTGTTCGA CGGCAAAAAGATCACTGTAACAGGGACCCTGTGGAACGGCAACAAA ATTATCGACGAGCGCCTGATCAACCCCGACGGCTCCCTGCTGTTCC GAGTAACCATCAACGGAGTGACCGGCTGGCGGCTGTGCGAACGC ATTCTGGCGGACTACAAGGACCACGACGGTGACTACAAGGACCACG ACATCGACTACAAGGACGACGACGACAAGTAGTAAACGCGTTGACGA TGG-3′). .. To generate a destabilized version of the vector, an identical gene block was synthesized with the addition of the PEST sequence (Promega) 5′-AATTCTCACGGCTTTCCGCCTGAGGTTGAAGAGCAAGC CGCCGGTACATTGCCTATGTCCTGCGCACAAGAAAGCGGTATGGACC GGCACCCAGCCGCTTGTGCTTCAGCTCGATCAACGTC-3′ upstream of the stop codon. pENTR223 Gateway entry vectors for the kinases were obtained from Hahn/Root Labs Human Kinases ORF Kit59,60 ( Sequencing:Article Title: Inhibitors supercharge kinase turnover through native proteolytic circuits. Article Snippet: The pLEX305-ccdB-Nluc-3×Flag luminescent reporter vector was generated as a destination vector starting from pLEX_305-ccdB-dTAG destination vector (Addgene, 91798) by restriction digest with AgeI and MluI and T4 DNA ligation (NEB) of a synthesized gene block (TWIST) containing the Nluc sequence and a C-terminal 3×Flag tag (5′-CGG GCAAAACCGGTGTCTTCACACTCGAAGATTTCGTTGGGGACTGGCGA CAGACAGCCGGCTACAACCTGGACCAAGTCCTTGAACAGGGAGGTG TGTCCAGTTTGTTTCAGAATCTCGGGGTGTCCGTAACTCCGATCCAA AGGATTGTCCTGAGCGGTGAAAATGGGCTGAAGATCGACATCCATGTC ATCATCCCGTATGAAGGTCTGAGCGGCGACCAAATGGGCCAGATCGA AAAAATTTTTAAGGTGGTGTACCCTGTGGATGATCATCACTTTAAGG TGATCCTGCACTATGGCACACTGGTAATCGACGGGGTTACGCCGAAC ATGATCGACTATTTCGGACGGCCGTATGAAGGCATCGCCGTGTTCGA CGGCAAAAAGATCACTGTAACAGGGACCCTGTGGAACGGCAACAAA ATTATCGACGAGCGCCTGATCAACCCCGACGGCTCCCTGCTGTTCC GAGTAACCATCAACGGAGTGACCGGCTGGCGGCTGTGCGAACGC ATTCTGGCGGACTACAAGGACCACGACGGTGACTACAAGGACCACG ACATCGACTACAAGGACGACGACGACAAGTAGTAAACGCGTTGACGA TGG-3′). .. To generate a destabilized version of the vector, an identical gene block was synthesized with the addition of the PEST sequence (Promega) 5′-AATTCTCACGGCTTTCCGCCTGAGGTTGAAGAGCAAGC CGCCGGTACATTGCCTATGTCCTGCGCACAAGAAAGCGGTATGGACC GGCACCCAGCCGCTTGTGCTTCAGCTCGATCAACGTC-3′ upstream of the stop codon. pENTR223 Gateway entry vectors for the kinases were obtained from Hahn/Root Labs Human Kinases ORF Kit59,60 ( Recombinant:Article Title: The Number of Larval Molts Is Controlled by Hox in Caterpillars. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488-conjugated GFP polyclonal antibody Thermo Fisher Cat#A-21311; RRID: AB_221477 Normal goat serum Sigma-Aldrich Cat#G9023 Biological Samples A genomic DNA panel of B. mori, see Table S1 for details NBRP Silkworm (https://silkworm.nbrp.jp/ index_en.html) N/A Chemicals, Peptides, and Recombinant Proteins TRIzol Reagent Thermo Fisher Cat#15596018 RNeasy Plus Mini Kit QIAGEN Cat#74134 ReverTra Ace qPCR RT Master Mix with gDNA Remover TOYOBO Cat#FSQ-301 Thunderbird SYBR qPCR Mix TOYOBO Cat#QPS-201 mMESSAGE mMACHINE T7 ULTRA Transcription Kit Thermo Fisher Cat#AM1345 TaKaRa Ex Taq TaKaRa Cat#RR001A Methanol Wako Cat#131-01826 alpha-Ecdysone Cayman Chemical Cat#11711 Rhodamine Phalloidin Thermo Fisher Cat#R415 Critical Commercial Assays 20-Hydroxyecdysone EIA Kit Bertin Bioreagent Cat#A05120 Experimental Models: Organisms/Strains B. mori strain p50 (+M) NBRP Silkworm (https://silkworm.nbrp.jp/ index_en.html) p50, a nearly isogenic strain used for whole genome sequencing35 B. mori strain pnd w-1 (+M) Tamura et al.36 N/A, a standard strain for transgenesis B. mori strain Kinshu x Showa (+M) Ueda Sanshu N/A, a commercial hybrid strain used for sericulture B. mori strain Shisen-sanmin (M3) NARO Genebank (https://www.gene.affrc. go.jp/index_en.php) ANJP10282 B. mori strain Kyudai-gomin (M5) NARO Genebank (https://www.gene.affrc. go.jp/index_en.php) ANJP10289 D. melanogaster y w, P[y[+].nos-int.NLS]; P [CaryP]attP2 Gift from Diogo Manoel37 N/A D. melanogaster y w; UAS-mCD8::GFP Bloomington Drosophila Stock Center #5137, RRID:BDSC_5137 Oligonucleotides See Table S2 for primers used in this study N/A Recombinant DNA Golden Gate TALEN and TAL effector kit 2.0 Cermak et al.38 Real-time Polymerase Chain Reaction:Article Title: The Number of Larval Molts Is Controlled by Hox in Caterpillars. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488-conjugated GFP polyclonal antibody Thermo Fisher Cat#A-21311; RRID: AB_221477 Normal goat serum Sigma-Aldrich Cat#G9023 Biological Samples A genomic DNA panel of B. mori, see Table S1 for details NBRP Silkworm (https://silkworm.nbrp.jp/ index_en.html) N/A Chemicals, Peptides, and Recombinant Proteins TRIzol Reagent Thermo Fisher Cat#15596018 RNeasy Plus Mini Kit QIAGEN Cat#74134 ReverTra Ace qPCR RT Master Mix with gDNA Remover TOYOBO Cat#FSQ-301 Thunderbird SYBR qPCR Mix TOYOBO Cat#QPS-201 mMESSAGE mMACHINE T7 ULTRA Transcription Kit Thermo Fisher Cat#AM1345 TaKaRa Ex Taq TaKaRa Cat#RR001A Methanol Wako Cat#131-01826 alpha-Ecdysone Cayman Chemical Cat#11711 Rhodamine Phalloidin Thermo Fisher Cat#R415 Critical Commercial Assays 20-Hydroxyecdysone EIA Kit Bertin Bioreagent Cat#A05120 Experimental Models: Organisms/Strains B. mori strain p50 (+M) NBRP Silkworm (https://silkworm.nbrp.jp/ index_en.html) p50, a nearly isogenic strain used for whole genome sequencing35 B. mori strain pnd w-1 (+M) Tamura et al.36 N/A, a standard strain for transgenesis B. mori strain Kinshu x Showa (+M) Ueda Sanshu N/A, a commercial hybrid strain used for sericulture B. mori strain Shisen-sanmin (M3) NARO Genebank (https://www.gene.affrc. go.jp/index_en.php) ANJP10282 B. mori strain Kyudai-gomin (M5) NARO Genebank (https://www.gene.affrc. go.jp/index_en.php) ANJP10289 D. melanogaster y w, P[y[+].nos-int.NLS]; P [CaryP]attP2 Gift from Diogo Manoel37 N/A D. melanogaster y w; UAS-mCD8::GFP Bloomington Drosophila Stock Center #5137, RRID:BDSC_5137 Oligonucleotides See Table S2 for primers used in this study N/A Recombinant DNA Golden Gate TALEN and TAL effector kit 2.0 Cermak et al.38 Enzyme-linked Immunosorbent Assay:Article Title: The Number of Larval Molts Is Controlled by Hox in Caterpillars. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488-conjugated GFP polyclonal antibody Thermo Fisher Cat#A-21311; RRID: AB_221477 Normal goat serum Sigma-Aldrich Cat#G9023 Biological Samples A genomic DNA panel of B. mori, see Table S1 for details NBRP Silkworm (https://silkworm.nbrp.jp/ index_en.html) N/A Chemicals, Peptides, and Recombinant Proteins TRIzol Reagent Thermo Fisher Cat#15596018 RNeasy Plus Mini Kit QIAGEN Cat#74134 ReverTra Ace qPCR RT Master Mix with gDNA Remover TOYOBO Cat#FSQ-301 Thunderbird SYBR qPCR Mix TOYOBO Cat#QPS-201 mMESSAGE mMACHINE T7 ULTRA Transcription Kit Thermo Fisher Cat#AM1345 TaKaRa Ex Taq TaKaRa Cat#RR001A Methanol Wako Cat#131-01826 alpha-Ecdysone Cayman Chemical Cat#11711 Rhodamine Phalloidin Thermo Fisher Cat#R415 Critical Commercial Assays 20-Hydroxyecdysone EIA Kit Bertin Bioreagent Cat#A05120 Experimental Models: Organisms/Strains B. mori strain p50 (+M) NBRP Silkworm (https://silkworm.nbrp.jp/ index_en.html) p50, a nearly isogenic strain used for whole genome sequencing35 B. mori strain pnd w-1 (+M) Tamura et al.36 N/A, a standard strain for transgenesis B. mori strain Kinshu x Showa (+M) Ueda Sanshu N/A, a commercial hybrid strain used for sericulture B. mori strain Shisen-sanmin (M3) NARO Genebank (https://www.gene.affrc. go.jp/index_en.php) ANJP10282 B. mori strain Kyudai-gomin (M5) NARO Genebank (https://www.gene.affrc. go.jp/index_en.php) ANJP10289 D. melanogaster y w, P[y[+].nos-int.NLS]; P [CaryP]attP2 Gift from Diogo Manoel37 N/A D. melanogaster y w; UAS-mCD8::GFP Bloomington Drosophila Stock Center #5137, RRID:BDSC_5137 Oligonucleotides See Table S2 for primers used in this study N/A Recombinant DNA Golden Gate TALEN and TAL effector kit 2.0 Cermak et al.38 Software:Article Title: The Number of Larval Molts Is Controlled by Hox in Caterpillars. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488-conjugated GFP polyclonal antibody Thermo Fisher Cat#A-21311; RRID: AB_221477 Normal goat serum Sigma-Aldrich Cat#G9023 Biological Samples A genomic DNA panel of B. mori, see Table S1 for details NBRP Silkworm (https://silkworm.nbrp.jp/ index_en.html) N/A Chemicals, Peptides, and Recombinant Proteins TRIzol Reagent Thermo Fisher Cat#15596018 RNeasy Plus Mini Kit QIAGEN Cat#74134 ReverTra Ace qPCR RT Master Mix with gDNA Remover TOYOBO Cat#FSQ-301 Thunderbird SYBR qPCR Mix TOYOBO Cat#QPS-201 mMESSAGE mMACHINE T7 ULTRA Transcription Kit Thermo Fisher Cat#AM1345 TaKaRa Ex Taq TaKaRa Cat#RR001A Methanol Wako Cat#131-01826 alpha-Ecdysone Cayman Chemical Cat#11711 Rhodamine Phalloidin Thermo Fisher Cat#R415 Critical Commercial Assays 20-Hydroxyecdysone EIA Kit Bertin Bioreagent Cat#A05120 Experimental Models: Organisms/Strains B. mori strain p50 (+M) NBRP Silkworm (https://silkworm.nbrp.jp/ index_en.html) p50, a nearly isogenic strain used for whole genome sequencing35 B. mori strain pnd w-1 (+M) Tamura et al.36 N/A, a standard strain for transgenesis B. mori strain Kinshu x Showa (+M) Ueda Sanshu N/A, a commercial hybrid strain used for sericulture B. mori strain Shisen-sanmin (M3) NARO Genebank (https://www.gene.affrc. go.jp/index_en.php) ANJP10282 B. mori strain Kyudai-gomin (M5) NARO Genebank (https://www.gene.affrc. go.jp/index_en.php) ANJP10289 D. melanogaster y w, P[y[+].nos-int.NLS]; P [CaryP]attP2 Gift from Diogo Manoel37 N/A D. melanogaster y w; UAS-mCD8::GFP Bloomington Drosophila Stock Center #5137, RRID:BDSC_5137 Oligonucleotides See Table S2 for primers used in this study N/A Recombinant DNA Golden Gate TALEN and TAL effector kit 2.0 Cermak et al.38 |